Monitoring the glucose level as well as alteration in expression of Research Proposal

Monitoring the glucose aim as well as alteration in expression of m-RNA and protien levels of glucose transporters(GLUT4) in d - Research Proposal ExampleThe reduction of insulin use removes the glucose transporter in the urine samples out of the body. This concludes that glucose level monitoring and the Vitamin D level monitoring is truly important for the type 2 Diabetes patients.1 When the Vitamin D binds to the vitamin D receptor (VDR), a heterodimer is formed. This hetereodimer then binds to the deoxyribonucleic acid and initiates some(prenominal) cofactors leading to the increase in the transcription of the genes that are responsible for the production of the proteins that control the calcium homeostasis. Vitamin D regulates the insulin receptor gene expression at the genetic level. The mRNA that codes for insulin receptor gene is produced at cock-a-hoop numbers by increasing the expression level. The increase in the insulin receptor will increase the back of the insulin and makes the GLUT4 transporter gene translocation from the intracellular level to the plasma membrane. 2 This increase in flux increases the glucose metabolic process and thus controls the type 2 Diabetes mellitus in humans.GLUT 4 contain the phosphate at the basal state. The phosphorylation of GLUT4 is negotiate by the cyclic cAMP- dependent protein kinase inhibiting the glucose transport.2 Scientific Background A study was carried out in the division 1993, to identify the genes that are responsible for the regulatory domain of the GLUT4 in the adipose tissue. The transcriptional regulation of the gene was analyze in the murine C2C12 skeletal muscle cell line. Transient transfection of the 5 and 3 deletions of the GLUT4 5 flanking DNA has identified a region of 281 base pairs present at the myotube-specific expression. 3 The total RNA was dislocated and used for Northern Blot analysis and hybridization was carried out. This gene was then inserted into the luciferase EcoRI- Dra I fragment plasmid and shape up analysis was carried out. 3 The GLUT4 mRNA was found to be dependent on the thyroid hormone. This region was likewise found between the -517 and -237 region. This 281 base pair region is found to play a major use in understanding the mechanism that controls the GLUT4 gene expression in the skeletal muscle. All these experiments were carried out in the rat model.3 The vitamin D deficiency is found to be associated with the impaired insulin secretion. When Vitamin D is supplied at sufficient level then it binds to the vitamin D receptors (VDRs) present in the pancreatic beta cells. VRD gene is present in the Chromosome 12-cen-q12. When allelic variations are done in the VDR receptor gene then glucose absorbance rate also varies. The genomic DNA was extracted from the quatern healthy individuals and the four SNPS at the intron 8 and Exon 9 was examined with the controls. 4 The peripheral squanderer samples were analyzed for the given SNPS at the VD R gene using the polymerase chain reaction and then by the restraint fragment length polymorphism (RFLP). The restriction sites looked for were TaqI, ApaI, BsmI and Tru91SNP. 4 It was found that the genotypes were similar in both the patients and control. This reason that VDR is not the major gene for the Type 2 Diabetes mellitus. Hence further research is necessary for conclusion the responsible gene for Type 3 Diabetes mellitus.4 The vitamin D is not only the factor that is responsible for the insulin resistance. at that place are other factors too. The 25- hydroxyl vitamin D (25(OH)-D) is found to be inversely proportional to the insulin resistance and the (25( OH)-D) preoccupation is determined in the serum for the analysis. The association between the